生物大分子重点实验室

1986 - 1990    南开大学，理学学士学位

1990 - 1993    北京师范大学，理学硕士学位

1993 - 1996    北京大学，理学博士学位

1996 - 1998    金沙集团1862cc，助理研究员

1998 - 2000 金沙集团1862cc副研究员，获得英国皇家学会K. C. Wong Fellowship，受英国皇家学会Joint project资助，在英国国立食品研究所（Institute of Food Research,　UK）做访问学者。

2000 - 至今    金沙集团1862cc创新研究课题组长(PI)，2004年晋升研究员

2004.12-2005.3 英国剑桥MRC（Medical Research Council），访问学者

2015 - 至今    生物大分子与科教融合卓越中心，研究员

2015 - 至今    金沙集团1862cc大学，岗位教授

2018.3 - 2018.8  美国国立卫生研究院 （NIH）国家神经疾病和中风研究所访问学者

2015 获中华人民共和国国务院“政府特殊津贴”

2014 金沙集团1862cc京区“巾帼建功”先进个人

2012 获国家杰出青年科学基金

2006 获第13届国际自由基大会青年研究者奖(亚洲)

　　生命离不开氧，细胞的氧化还原状态直接调控蛋白质等大分子功能，氧化还原应激是细胞信号转导和效应的重要调控方式，介导衰老、神经退行性疾病、代谢病、肿瘤等许多生理和病理过程。陈畅课题组通过研究生物大分子特异氧化还原修饰，尤其是巯基亚硝基化修饰的研究，回答了细胞氧化还原调控的特异性问题，并阐释了氧化应激的信号作用分子机制和生理功能，揭示了亚硝基化修饰调控"年老忘事"和"中年发福"的新机制；提出精准氧化还原概念和"5R"精准氧化还原调控原则（Right time/place/level/target/species），破解了半个多世纪以来抗氧化药物研发僵局，开启精准氧化还原医药研发；提出了"通过增大氧化还原信号阈值RST、提高氧化还原应激响应能力RRC、延缓氧化还原应激响应抵抗RRR"的主动抗衰老主动健康策略，提出"抗衰老要从小做起"；系统解析了《本草纲目》记载的枸杞子 "久服坚筋骨，轻身不老，耐寒暑，易颜色，变白" 传统功效的科学内涵和分子机制，获得了明确靶点、明确有效成分、明确分子机制的衰老干预策略。

（标注*为通讯作者）

1. Yang Q^#, Yan R^#, Zhang J^#, Zhang T, Kong Q, Zhang X, Xia H, Ye A, Qiao X, Kato K, Chen C*, An Y*. Reductive stress induced by NRF2/G6PD through glucose metabolic reprogramming promotes malignant transformation in Arsenite-exposed human keratinocytes. Sci Total Environ. 2023 Oct 20;896:165207.

2. Lv Z^#, Meng J^#, Yao S^#, Xiao F^#, Li S, Shi H, Cui C, Chen K, Luo X*, Ye Y*, Chen C*. Naringenin improves muscle endurance via activation of the Sp1-ERRγ transcriptional axis. Cell Reports. 2023 Oct 23;42(11):113288.

3. Qiao, X^#, Kang, L^#, Shi, C^#, Ye A^#, Wu D, Huang Y, Deng M, Wang J, Zhao Y*, Chen C*. Exploring the precision redox map during fasting-refeeding and satiation in C. elegans. Stress Biology. 2023 3, 17.

4. Guo M^#, Qiao X^#, Wang Y^#, Li Z^#, Shi C, Chen Y, Kang L, Chen C*, Zhou X*. Mitochondrial translational defect extends lifespan in C. elegans by activating UPRmt. Redox Biol. 2023 July;63:102722.

5. Meng J, Wang Y, Lv Z, Qiao X, Ye A, Zhu Q, Chen C*. Redox-stress response resistance (RRR) mediated by hyperoxidation of peroxiredoxin 2 in senescent cells. Sci China Life Sci. 2023 Oct;66(10):2280-2294.

6. Meng J^#, Lv Z^#, Chen X^#, Sun C, Jin C, Ding K*, Chen C*. LBP1C-2 from Lycium barbarum maintains skeletal muscle satellite cell pool by interaction with FGFR1. iScience. 2023, 106573.

7. Zhang H^#, Yu F^#, Xie P^#, Sun S^#, Qiao X^#, Tang S, Chen C, Yang S, Mei C, Yang D, Wu Y, Xia R, Li X, Lu J, Liu Y, Xie X, Ma D, Xu X, Liang Z, Feng Z, Huang X, Yu H, Liu G, Wang Y, Li J, Zhang Q, Chen C, Ouyang Y, Xie Q*. A Gγ protein regulates alkaline sensitivity in crops. Science. 2023 Mar 24;379(6638):eade8416.

8. Sun C^#, Chen X^#, Yang S, Jin C, Ding K*, Chen C*. LBP1C-2 from Lycium barbarum alleviated age-related bone loss by targeting BMPRA/BMPRII/Noggin. Carbohyd Polym. 2023, 310: 120725.

9. Zhang W, Qiao X，Xie T, Cai W, Zhang X, Chen C*, Zhang Y. Multi-Omics Approach Reveals Redox Homeostasis Reprogramming in Early-Stage Clear Cell Renal Cell Carcinoma. Antioxidants. 2023, 12, 81.

10. Gao L^#, Qiao X^#, Zhang L, Wang Y, Wan Y*, Chen C*. Nitric oxide inhibits alginate biosynthesis in Pseudomonas aeruginosa and increases its sensitivity to tobramycin by downregulating algU gene expression. Nitric Oxide. 2022 Aug 17:S1089-8603(22)00084-2.

11. 陈畅*. 精准氧化还原调控. 药学学报. 2022, 57(6): 1541-1543.

12. Zhang Y^#, Qiao X^#,*, Liu L^#, Han W, Liu Q, Wang Y, Xie T, Tang Y, Wang T, Meng J, Ye A, He S, Chen R*, Chen C*. Long noncoding RNA MAGI2-AS3 regulates the H₂O₂ level and cell senescence via HSPA8. Redox Biol. 2022 Aug;54:102383.

13. Wang Y^#, Qiao X^#, Shi C^#, Ye A^#, Guo M, Zhao Y*, Chen C*. Exercise Alleviates ER Reductive Stress and Promotes Healthy Aging[J]. Prog Biochem Biophys. 2022, 49(3): 444-453.

14. Meng J^#, Lv Z^#, Guo M, Sun C, Li X, Jiang Z, Zhang W, Chen C*. A Lycium barbarum extract inhibits β-amyloid toxicity by activating the antioxidant system and mtUPR in a Caenorhabditis elegans model of Alzheimer's disease. FASEB J. 2022 Feb;36(2):e22156.

15. Qiao X, Zhang Y, Ye A, Zhang Y, Xie T, Lv Z, Shi C, Wu D, Chu B, Wu X, Zhang W, Wang P, Liu GH, Wang CC, Wang L*, Chen C*. ER reductive stress caused by Ero1α S-nitrosation accelerates senescence. Free Radic Biol Med. 2022 Feb 20;180:165-178.

16. Xie T^#, Qiao X^#, Sun C^#, Chu B, Meng J, Chen C*. GAPDH S-nitrosation contributes to age-related sarcopenia through mediating apoptosis. Nitric Oxide. 2021 Dec 29;120:1-8.

17. Meng J, Lv Z, Wang Y, Chen C*. Identification of the redox-stress signaling threshold (RST): Increased RST helps to delay aging in C. elegans. Free Radic Biol Med. 2021 Nov 27;178:54-58.

18. Cai WB, Zhao YJ, Liu L, Cheng Q, Wang J, Shi XL, Yao L, Qiao XH, Zhu Y, Chen C*, Zhang X*. Redox environment metabolomic evaluation (REME) of the heart after myocardial ischemia/reperfusion injury. Free Radic Biol Med. 2021 Jul 9;173:7-18.

19. Yi W^#, Zhang Y^#, Liu B^#,*, Zhou Y, Liao D, Qiao X, Gao D, Xie T, Yao Q, Zhang Y, Qiu Y, Huang G, Chen Z*, Chen C*, Ju Z*. Protein S-nitrosylation regulates proteostasis and viability of hematopoietic stem cell during regeneration. Cell Rep. 2021 Mar 30;34(13):108922.

20. Meng J, Lv Z, Zhang Y, Wang Y, Qiao X, Sun C, Chen Y, Guo M, Han W, Ye A, Xie T, Chu B, Shi C, Yang S, Chen C*. Precision Redox: The Key for Antioxidant Pharmacology. Antioxid Redox Signal. 2021 May 10;34(14):1069-1082.

21. Meng J^#, Lv Z^#, Sun C, Qiao X, Chen C*. An extract of Lycium barbarum mimics exercise to improve muscle endurance through increasing type IIa oxidative muscle fibers by activating ERRγ. FASEB J. 2020 Sep;34(9):11460-11473.

22. Yang J^#, Zhang H^#, Gong W^#, Liu Z, Wu H, Hu W, Chen X, Wang L, Wu S, Chen C*, Perrett S*. S-Glutathionylation of human inducible Hsp70 reveals a regulatory mechanism involving the C-terminal α-helical lid. J Biol Chem. 2020 Jun 12;295(24):8302-8324.

23. Zheng WQ^#, Zhang Y^#, Yao Q^#, Chen Y^#, Qiao X, Wang ED*, Chen C*, Zhou XL*. Nitrosative stress inhibits aminoacylation and editing activities of mitochondrial threonyl-tRNA synthetase by S-nitrosation. Nucleic Acids Res. 2020 Jul 9;48(12):6799-6810.

24. Ban Y^#, Liu Y^#, Li Y, Zhang Y, Xiao L, Gu Y, Chen S, Zhao B, Chen C*, Wang N*. S-nitrosation impairs KLF4 activity and instigates endothelial dysfunction in pulmonary arterial hypertension. Redox Biol. 2019 Feb;21:101099.

25. Li J, Zhang Y, Zhang Y, Lü S, Miao Y, Yang J, Huang S, Ma X, Han L, Deng J, Fan F, Liu B, Huo Y, Xu Q, Chen C*, Wang X*, Feng J*. GSNOR modulates hyperhomocysteinemia-induced T cell activation and atherosclerosis by switching Akt S-nitrosylation to phosphorylation. Redox Biol. 2018 Jul;17:386-399.

26. Li J, Gao Z, Zhao D, Zhang L, Qiao X, Zhao Y, Ding H, Zhang P, Lu J, Liu J, Jiang H, Luo C*, Chen C*. PI-273, a Substrate-Competitive, Specific Small-Molecule Inhibitor of PI4KIIα, Inhibits the Growth of Breast Cancer Cells. Cancer Res. 2017 Nov 15;77(22):6253-6266.

27. Zhang Y, Wu K, Su W, Zhang DF, Wang P, Qiao X, Yao Q, Yuan Z, Yao YG, Liu G, Zhang C, Liu L, Chen C*. Increased GSNOR Expression during Aging Impairs Cognitive Function and Decreases S-Nitrosation of CaMKIIα. J Neurosci. 2017 Oct 4;37(40):9741-9758.

28. Li Y, Zhang Y, Wang L, Wang P, Xue Y, Li X, Qiao X, Zhang X, Xu T, Liu G, Li P, Chen C*. Autophagy impairment mediated by S-nitrosation of ATG4B leads to neurotoxicity in response to hyperglycemia. Autophagy. 2017 Jul 3;13(7):1145-1160.

29. Ding Y^#, Li Y^#, Zhang X, He J, Lu D, Fang X, Wang Y, Wang J, Zhang Y, Qiao X, Gan LM, Chen C*, Zhu Y*. Soluble epoxide hydrolase activation by S-nitrosation contributes to cardiac ischemia-reperfusion injury. J Mol Cell Cardiol. 2017 Sep;110:70-79.

30. Meng J, Lv Z, Qiao X, Li X, Li Y, Zhang Y, Chen C*. The decay of Redox-stress Response Capacity is a substantive characteristic of aging: Revising the redox theory of aging. Redox Biol. 2017 Apr;11:365-374.

31. Zhang H, Yang J, Wu S, Gong W, Chen C*, Perrett S*. Glutathionylation of the Bacterial Hsp70 Chaperone DnaK Provides a Link between Oxidative Stress and the Heat Shock Response. J Biol Chem. 2016 Mar 25;291(13):6967-81.

32. Gao L^#, Zhang Y^#, Wang Y, Qiao X, Zi J, Chen C*, Wan Y*. Reduction of PCN biosynthesis by NO in Pseudomonas aeruginosa. Redox Biol. 2016 Aug;8:252-8.

33. Yin R, Fang L, Li Y, Xue P, Li Y, Guan Y, Chang Y, Chen C*, Wang N*. Pro-inflammatory Macrophages suppress PPARγ activity in Adipocytes via S-nitrosylation. Free Radic Biol Med. 2015 Dec;89:895-905.

34. Qiao, X, HU, M, Zhang, Y, Li Y, Chen, C*. Cellular redox regulation and thiol modification. Chinese Bulletin of Life Sciences. 2015 27(3), 374-382.

35. Zhou Q^#, Li J^#, Yu H, Zhai Y, Gao Z, Liu Y, Pang X, Zhang L, Schulten K*, Sun F*, Chen C*. Molecular insights into the membrane-associated phosphatidylinositol 4-kinase IIα. Nat Commun. 2014 Mar 28;5:3552.

36. Li J, Zhang L, Gao Z, Kang H, Rong G, Zhang X, Chen C*. Dual inhibition of EGFR at protein and activity level via combinatorial blocking of PI4KIIα as anti-tumor strategy. Protein Cell. 2014 Jun;5(6):457-68.

37. Sun F, Li J, Chen C*. Puzzle out the regulation mechanism of PI4KIIα activity. Sci China Life Sci. 2014 Jun;57(6):636-8.

38. Wu K^#, Ren R^#, Su W^#, Wen B, Zhang Y, Yi F, Qiao X, Yuan T, Wang J, Liu L, Izpisua Belmonte JC*, Liu GH*, Chen C*. A novel suppressive effect of alcohol dehydrogenase 5 in neuronal differentiation. J Biol Chem. 2014 Jul 18;289(29):20193-9.

39. Wu K, Zhang Y, Wang P, Zhang L, Wang T, Chen C*. Activation of GSNOR transcription by NF-κB negatively regulates NGF-induced PC12 differentiation. Free Radic Res. 2014 Sep;48(9):1011-7.

40. Hu M, Xu T*, Chen C*. Evaluation of the mitochondrial redox status in Caenorhabditis elegans: an assay based on the COPAS biosort flow cytometer. Acta Biophys Sin. 2014 30(2), 110-116.

41. Mao K^#, Chen S^#, Chen M, Ma Y, Wang Y, Huang B, He Z, Zeng Y, Hu Y, Sun S, Li J, Wu X, Wang X, Strober W, Chen C*, Meng G*, Sun B*. Nitric oxide suppresses NLRP3 inflammasome activation and protects against LPS-induced septic shock. Cell Res. 2013 Feb;23(2):201-12.

42. Zhang X^#, Huang B^#, Zhang L, Zhang Y, Zhao Y, Guo X, Qiao X, Chen C*. SNObase, a database for S-nitrosation modification. Protein Cell. 2012 Dec;3(12):929-33.

43. Zhang X^#, Huang B^#, Chen C*. SNO spectral counting (SNOSC), a label-free proteomic method for quantification of changes in levels of protein S-nitrosation. Free Radic Res. 2012 Aug;46(8):1044-50.

44. Huang B, Chen C*. Function and Mechanism of Nitric Oxide (I)-Characteristics and Function. Acta Biophys Sin. 2012 28(3), 173-184.

45. Huang B, Chen C*.??Function and Mechanism of Nitric Oxide (II)-Mechanism and Protein S-Nitrosation. Acta Biophys Sin. 2012 28(4), 268-277.

46. Hou Q^#, Jiang H^#, Zhang X, Guo C, Huang B, Wang P, Wang T, Wu K, Li J, Gong Z, Du L, Liu Y, Liu L*, Chen C*. Nitric oxide metabolism controlled by formaldehyde dehydrogenase (fdh, homolog of mammalian GSNOR) plays a crucial role in visual pattern memory in Drosophila. Nitric Oxide. 2011 Jan 1;24(1):17-24.

47. Zhang X^#, Huang B^#, Zhou X, Chen C*. Quantitative proteomic analysis of S-nitrosated proteins in diabetic mouse liver with ICAT switch method. Protein Cell. 2010 Jul;1(7):675-87.

48. Huang B, Chen C*. Detection of protein S-nitrosation using irreversible biotinylation procedures (IBP). Free Radic Biol Med. 2010 Aug 1;49(3):447-56.

49. Zhou X, Han P, Li J, Zhang X, Huang B, Ruan HQ, Chen C*. ESNOQ, proteomic quantification of endogenous S-nitrosation. PLoS One. 2010 Apr 2;5(4):e10015.

50. Li J, Lu Y, Zhang J, Kang H, Qin Z*, Chen C*. PI4KIIalpha is a novel regulator of tumor growth by its action on angiogenesis and HIF-1alpha regulation. Oncogene. 2010 Apr 29;29(17):2550-9.

专      利：

1. Chang Chen, Kan Ding, Xingke Yang, Chuanxin Sun, Xia Chen, Can Jin，LBP1C-2, a homogenous polysaccharide from Lycium barbarum, methods of using in age-related bone loss. Patent Application No. PCT/IB2023/000414.

2. Chang Chen, Jiao Meng, Zhenyu Lv, Chuanxin Sun, Xinhua Qiao, LYCIUMBARBARUMEXTRACTS,RESULTINGCOMPOSITIONS,METHODSOF MAKING ANDMETHODSOF USING THE SAME. Patent Application No. 42021042963.5 （香港）

3. Chang Chen, Kan Ding, Xingke Yang, Jiao Meng, Can Jin, Zhenyu Lv, Chuanxin Sun, Xia Chen.COMPOSITIONCOMPRISINGHOMOGENEOUSPOLYSACCHARIDEAND METHOD OF USING THE SAME TOIMPROVEMUSCLESATELLITE CELLS. Patent Application NO.PCT/IB2023/000278.

4. Chang Chen, Yang Ye, Xingke Yang, Kaixian Chen, Xiaomin Luo, Jiao Meng, Sheng Yao, Zhenyu Lv.COMPOSITIONCOMPRISINGNARINGENIN ORDERIVATIVETHEREOF, METHOD OF MAKING AND METHOD OF USING THE SAME. Patent Application NO.PCT/IB2023/000179.

5. 陈畅；孟姣；吕振宇；孙传鑫；乔新华，含枸杞提取物的组合物及其制备方法和应用方法，ZL202110023756.8 授权日期：2022-01-08

6. Chang Chen, Jiao Meng, Zhenyu Lv, Chuanxin Sun, Xinhua Qiao, Lycium barbarum extracts, resulting compositions, methods of making and methods of using the same. Patent No. US11,135,260 B2, Date of Patent: 2021-10-05.

7. 仝小林；陈畅，一种抗抑郁的药物组合物和制备方法及其用途，ZL201811628807.4，授权日期：2021-12-17。

8. 仝小林；陈畅，一种治疗糖尿病的药物组合物和制备方法及其用途，ZL 201811626580.X，授权日期：2021-10-22，授权公告号：CN111375020B。

9. 陈畅、罗成、蒋华良、李江美、高振、赵丹、张伦峰、乔新华，肌醇磷脂4位激酶二型α亚型特异抑制剂PI-273的应用，ZL 201510831539.6, 授权日期：2021-1-29

10. Yi Wan, Chang Chen, Lei Gao, Yuying Zhang, Xinhua Qiao, Yan Wang, Method, target and application for NO accumulation to decrease Pseudomonas aeruginosa invasiveness，US 10537593B2, United States，授权日期：2020-01-21

11. 万一；陈畅；高振；张玉英；乔新华；王琰，NO累计降低铜绿假单胞菌侵袭力的方法、靶点及应用，ZL 201510697439.9，授权日期：2019-5-17

12. 陈畅、黄波，特异检测蛋白质或多肽半胱氨酸巯基修饰的方法及其用途，ZL200910084155.7，授权日期：2013-08-28，授权公告号：CN101893634B。

13. 陈畅、李江美，肌醇磷脂4位激酶二型α亚型 PI4KIIα的应用，ZL200810104272.0，授权日期：2013-05-08，授权公告号：CN101560546B。

论著章节：

1. Chang Chen, Free Radical Biology. Encyclopedia of China, 2017.

2. Chang　Chen,　H.　R.　Tang,　and　P.　Belton.　Natural　antioxidants　-　an　ESR　perspective,　in　"Magnetic　Resonance　in　Food　Science”,　Ed.　G.A.　Webb,　The　Royal　Society　of　Chemistry　publishing,　UK.　2000.

3. 陈畅，“一氧化氮功能及其作用机制”，《自由基生物医学》第七章，主编：郑荣梁、赵崇义、魏耀挥、黄中洋，艺轩图书出版社，台湾，2013，ISBN　978-957-616-983-0.

（资料来源：陈畅研究员，2023-12-08）