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Molecular-scale axial localization by repetitive optical selective exposure, Nat Methods, 1 Apr 2021

发布时间:2021年04月01日

Nature Methods, 11 April, 2021, DOI:https://dx.doi.org/10.1038/s41592-021-01099-2

Molecular-scale axial localization by repetitive optical selective exposure

Lusheng Gu, Yuanyuan Li, Shuwen Zhang, Maoge Zhou, Yanhong Xue, Weixing Li, Tao Xu & Wei Ji

Abstract

We introduce an axial localization with repetitive optical selective exposure (ROSE-Z) method for super-resolution imaging. By using an asymmetric optical scheme to generate interference fringes, a <2 nm axial localization precision was achieved with only ~3,000 photons, which is an approximately sixfold improvement compared to previous astigmatism methods. Nanoscale three-dimensional and two-color imaging was demonstrated, illustrating how this method achieves superior performance and facilitates the investigation of cellular nanostructures.

文章链接:https://www.nature.com/articles/s41592-021-01099-2

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