Di Li, Huw Colin-York, Liliana Barbieri, Yousef Javanmardi, Yuting Guo, Kseniya Korobchevskaya, Emad Moeendarbary, Dong Li & Marco Fritzsche
Abstract
Quantifying small, rapidly progressing three-dimensional forces generated by cells remains a major challenge towards a more complete understanding of mechanobiology. Traction force microscopy is one of the most broadly applied force probing technologies but ascertaining three-dimensional information typically necessitates slow, multi-frame z-stack acquisition with limited sensitivity. Here, by performing traction force microscopy using fast single-frame astigmatic imaging coupled with total internal reflection fluorescence microscopy we improve the temporal resolution of three-dimensional mechanical force quantification up to 10-fold compared to its related super-resolution modalities. 2.5D astigmatic traction force microscopy (aTFM) thus enables live-cell force measurements approaching physiological sensitivity.
最新重要论文
Astigmatic traction force microscopy (aTFM), Nat Commun, 12 Apr 2021
Nature Communications, 12 April, 2021, DOI:https://doi.org/10.1038/s41467-021-22376-w
Astigmatic traction force microscopy (aTFM)
Di Li, Huw Colin-York, Liliana Barbieri, Yousef Javanmardi, Yuting Guo, Kseniya Korobchevskaya, Emad Moeendarbary, Dong Li & Marco Fritzsche
Abstract
Quantifying small, rapidly progressing three-dimensional forces generated by cells remains a major challenge towards a more complete understanding of mechanobiology. Traction force microscopy is one of the most broadly applied force probing technologies but ascertaining three-dimensional information typically necessitates slow, multi-frame z-stack acquisition with limited sensitivity. Here, by performing traction force microscopy using fast single-frame astigmatic imaging coupled with total internal reflection fluorescence microscopy we improve the temporal resolution of three-dimensional mechanical force quantification up to 10-fold compared to its related super-resolution modalities. 2.5D astigmatic traction force microscopy (aTFM) thus enables live-cell force measurements approaching physiological sensitivity.
文章链接:https://www.nature.com/articles/s41467-021-22376-w
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