Two-dimensional TIRF-SIM-traction force microscopy (2D TIRF-SIM-TFM)
Liliana Barbieri, Huw Colin-York, Kseniya Korobchevskaya, Di Li, Deanna L. Wolfson, Narain Karedla, Falk Schneider, Balpreet S. Ahluwalia, Tore Seternes, Roy A. Dalmo, Michael L. Dustin, Dong Li & Marco Fritzsche
Abstract
Quantifying small, rapidly evolving forces generated by cells is a major challenge for the understanding of biomechanics and mechanobiology in health and disease. Traction force microscopy remains one of the most broadly applied force probing technologies but typically restricts itself to slow events over seconds and micron-scale displacements. Here, we improve >2-fold spatially and >10-fold temporally the resolution of planar cellular force probing compared to its related conventional modalities by combining fast two-dimensional total internal reflection fluorescence super-resolution structured illumination microscopy and traction force microscopy. This live-cell 2D TIRF-SIM-TFM methodology offers a combination of spatio-temporal resolution enhancement relevant to forces on the nano- and sub-second scales, opening up new aspects of mechanobiology to analysis.
附件下载：
Two-dimensional TIRF-SIM-traction force microscopy (2D TIRF-SIM-TFM), Nat Commun, 12 Apr 2021
Nature Communications, 12 April, 2021, DOI：https://doi.org/10.1038/s41467-021-22377-9
Two-dimensional TIRF-SIM-traction force microscopy (2D TIRF-SIM-TFM)
Liliana Barbieri, Huw Colin-York, Kseniya Korobchevskaya, Di Li, Deanna L. Wolfson, Narain Karedla, Falk Schneider, Balpreet S. Ahluwalia, Tore Seternes, Roy A. Dalmo, Michael L. Dustin, Dong Li & Marco Fritzsche
Abstract
Quantifying small, rapidly evolving forces generated by cells is a major challenge for the understanding of biomechanics and mechanobiology in health and disease. Traction force microscopy remains one of the most broadly applied force probing technologies but typically restricts itself to slow events over seconds and micron-scale displacements. Here, we improve >2-fold spatially and >10-fold temporally the resolution of planar cellular force probing compared to its related conventional modalities by combining fast two-dimensional total internal reflection fluorescence super-resolution structured illumination microscopy and traction force microscopy. This live-cell 2D TIRF-SIM-TFM methodology offers a combination of spatio-temporal resolution enhancement relevant to forces on the nano- and sub-second scales, opening up new aspects of mechanobiology to analysis.
文章链接：https://www.nature.com/articles/s41467-021-22377-9
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