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In situ structures of polymerase complex of mammalian reovirus illuminate RdRp activation and transcription regulation, PNAS, 5 Dec 2022

发布时间:2022年12月05日

PNAS, 5 December, 2022, DOI:https://doi.org/10.1073/pnas.2203054119

In situ structures of polymerase complex of mammalian reovirus illuminate RdRp activation and transcription regulation

Keyan Bao, Xueli Zhang, Dongyu Li, Wei Sun, Zhenzhao Sun, Jingfei Wang, and Ping Zhu 

Abstract

Mammalian reovirus (reovirus) is a multilayered, turreted member of Reoviridae characterized by transcription of dsRNA genome within the innermost capsid shell. Here, we present high-resolution in situ structures of reovirus transcriptase complex in an intact double-layered virion, and in the uncoated single-layered core particles in the unloaded, reloaded, pre-elongation, and elongation states, respectively, obtained by cryo-electron microscopy and sub-particle reconstructions. At the template entry of RNA-dependent RNA polymerase (RdRp), the RNA-loading region gets flexible after uncoating resulting in the unloading of terminal genomic RNA and inactivity of transcription. However, upon adding transcriptional substrates, the RNA-loading region is recovered leading the RNAs loaded again. The priming loop in RdRp was found to play a critical role in regulating transcription, which hinders the elongation of transcript in virion and triggers the rearrangement of RdRp C-terminal domain (CTD) during elongation, resulting in splitting of template-transcript hybrid and opening of transcript exit. With the integration of these structures, a transcriptional model of reovirus with five states is proposed. Our structures illuminate the RdRp activation and regulation of the multilayered turreted reovirus.

文章链接:https://www.pnas.org/doi/10.1073/pnas.2203054119

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