当前位置:  首页 >> 最新重要论文

最新重要论文

Landscape of RNA pseudouridylation in archaeon Sulfolobus islandicus, Nucleic Acids Res, 20 Feb 2024

发布时间:2024年02月20日

Nucleic Acids Research, 20 February, 2024, DOI:https://doi.org/10.1093/nar/gkae096

Landscape of RNA pseudouridylation in archaeon Sulfolobus islandicus

Yuqian Li, Songlin Wu, Keqiong Ye

Abstract

Pseudouridine, one of the most abundant RNA modifications, is synthesized by stand-alone or RNA-guided pseudouridine synthases. Here, we comprehensively mapped pseudouridines in rRNAs, tRNAs and small RNAs in the archaeon Sulfolobus islandicus and identified Cbf5-associated H/ACA RNAs. Through genetic deletion and in vitro modification assays, we determined the responsible enzymes for these modifications. The pseudouridylation machinery in S. islandicus consists of the stand-alone enzymes aPus7 and aPus10, and six H/ACA RNA-guided enzymes that account for all identified pseudouridines. These H/ACA RNAs guide the modification of all eleven sites in rRNAs, two sites in tRNAs, and two sites in CRISPR RNAs. One H/ACA RNA shows exceptional versatility by targeting eight different sites. aPus7 and aPus10 are responsible for modifying positions 13, 54 and 55 in tRNAs. We identified four atypical H/ACA RNAs that lack the lower stem and the ACA motif and confirmed their function both in vivo and in vitro. Intriguingly, atypical H/ACA RNAs can be modified by Cbf5 in a guide-independent manner. Our data provide the first global view of pseudouridylation in archaea and reveal unexpected structures, substrates, and activities of archaeal H/ACA RNPs.

文章链接:https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae096/7611029

相关报道:https:/kyjz/zxdt/202402/t20240227_6995821.html


    附件下载: